| Sunday, April 4th, 2010 |
| Drafted 2B Tim McGee in the 2010 inaugural draft (Round 1, Pick 2, 2nd overall pick). |
| Drafted SP Luis Yánez in the 2010 inaugural draft (Round 2, Pick 2, 4th overall pick). |
| Drafted 3B Steve Rice in the 2010 inaugural draft (Round 3, Pick 2, 6th overall pick). |
| Drafted SP Ryan Woerman in the 2010 inaugural draft (Round 4, Pick 2, 8th overall pick). |
| Drafted RF Miguel Batista in the 2010 inaugural draft (Round 5, Pick 2, 10th overall pick). |
| Drafted CF Héctor Bernard in the 2010 inaugural draft (Round 6, Pick 2, 12th overall pick). |
| Drafted SP Ángel Salgado in the 2010 inaugural draft (Round 7, Pick 2, 14th overall pick). |
| Drafted CL Roberto Zamora in the 2010 inaugural draft (Round 8, Pick 2, 16th overall pick). |
| Drafted LF Keith Mathis in the 2010 inaugural draft (Round 9, Pick 2, 18th overall pick). |
| Drafted SP Alfredo Navarro in the 2010 inaugural draft (Round 10, Pick 2, 20th overall pick). |
| Drafted SS Ton Yamashita in the 2010 inaugural draft (Round 11, Pick 2, 22nd overall pick). |
| Drafted 1B Pedro Pérez in the 2010 inaugural draft (Round 12, Pick 2, 24th overall pick). |
| Drafted MR Chris McKinery in the 2010 inaugural draft (Round 13, Pick 2, 26th overall pick). |
| Drafted MR Pedro Márquez in the 2010 inaugural draft (Round 14, Pick 2, 28th overall pick). |
| Drafted SP Michel Sidotti in the 2010 inaugural draft (Round 15, Pick 2, 30th overall pick). |
| Drafted CL Salvo Fonzarelli in the 2010 inaugural draft (Round 16, Pick 2, 32nd overall pick). |
| Drafted MR Gerald Boyle in the 2010 inaugural draft (Round 17, Pick 2, 34th overall pick). |
| Drafted C Joe Bishop in the 2010 inaugural draft (Round 18, Pick 2, 36th overall pick). |
| Drafted C Yoshi Yamada in the 2010 inaugural draft (Round 19, Pick 2, 38th overall pick). |
| Drafted MR Hyung-on Ko in the 2010 inaugural draft (Round 20, Pick 2, 40th overall pick). |
| Drafted SS José Cruz in the 2010 inaugural draft (Round 21, Pick 2, 42nd overall pick). |
| Drafted CF Steve Burns in the 2010 inaugural draft (Round 22, Pick 2, 44th overall pick). |
| Drafted RF Manny Gutiérrez in the 2010 inaugural draft (Round 23, Pick 2, 46th overall pick). |
| Released 2B Tim McGee. |
| Released 1B Pedro Pérez. |
| Released C Joe Bishop. |
| Released SP Alfredo Navarro. |
| Released SP Ángel Salgado. |
| Released SP Michel Sidotti. |
| Released SP Ryan Woerman. |
| Released SP Luis Yánez. |
| Released MR Gerald Boyle. |
| Released MR Hyung-on Ko. |
| Released MR Pedro Márquez. |
| Released MR Chris McKinery. |
| Released CL Salvo Fonzarelli. |
| Released CL Roberto Zamora. |
| Released C Yoshi Yamada. |
| Released 3B Steve Rice. |
| Released SS Ton Yamashita. |
| Released SS José Cruz. |
| Released LF Keith Mathis. |
| Released CF Héctor Bernard. |
| Released CF Steve Burns. |
| Released RF Miguel Batista. |
| Released RF Manny Gutiérrez. |
| Placed SP Yovani Gallardo on the active roster. |
| Placed SP Roy Halladay on the active roster. |
| Placed SP Phil Hughes on the active roster. |
| Placed SP Ubaldo Jimenez on the active roster. |
| Placed SP Josh Johnson on the active roster. |
| Placed SP Jon Lester on the active roster. |
| Placed CL Heath Bell on the active roster. |
| Placed CL Rafael Soriano on the active roster. |
| Placed CL Billy Wagner on the active roster. |
| Placed C Mike Napoli on the active roster. |
| Placed C Victor Martinez on the active roster. |
| Placed 1B Albert Pujols on the active roster. |
| Placed 1B Miguel Cabrera on the active roster. |
| Placed 1B Paul Konerko on the active roster. |
| Placed 2B Dan Uggla on the active roster. |
| Placed 2B Rickie Weeks on the active roster. |
| Placed 3B David Wright on the active roster. |
| Placed SS Elvis Andrus on the active roster. |
| Placed LF Carl Crawford on the active roster. |
| Placed LF Ryan Braun on the active roster. |
| Placed CF Vernon Wells on the active roster. |
| Placed RF Vladimir Guerrero on the active roster. |
| Placed RF Shin-Soo Choo on the active roster. |
